IJE TRANSACTIONS B: Applications Vol. 31, No. 2 (February 2018) 188-195   

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E. Pashai, G. D. Najafpour, M. Jahanshahi and M. Rahimnejad
( Received: December 11, 2017 – Accepted in Revised Form: January 04, 2018 )

Abstract    A sensitive electrochemical sensor was fabricated for selective detection of nitric oxide (NO) based on electrochemically reduced graphene (ErGO)-polyaniline (PANI)-gold nanoparticles (AuNPs) nanocomposite. It was coated on a gold (Au) electrode through stepwise electrodeposition to form AuNPs-PANI-ErGO/Au electrode. The AuNPs-PANI-rGO nanocomposite was characterized by Field Emission Scanning Electron Microscopy (FESEM) and UV-vis. Electrochemical behavior of modified electrode was analyzed by cyclic voltammetry (CV) and chronoamperometry (CA) techniques. CVs of AuNPs-PANI-ErGO/Au, PANI-ErGO/Au and ErGO/Au electrodes showed that conductivity of AuNPs-PANI-ErGO/Au was higher than others. Nafion was used to improve selectivity of modified electrode. Nafion/AuNPs-PANI-ErGO/Au electrode represented favorable electrochemical and electrocatalytic behavior towards NO oxidation. The resultant electrode exhibited a high sensitivity of 0.113 μA/μM over a wide linear range from 0.8 × 10−6 to 86 × 10−6 M with a low detection limit of 2.5 × 10−7 M (S/N=3). In addition, the sensor had excellent stability, as well as reproducibility and selectivity, which makes it possible to detect NO quickly and accurately.


Keywords    Nitric oxide sensor, Reduced graphene oxide, Gold nanoparticle, Polyaniline, Cyclic voltammetry


چکیده    در این مطالعه، یک سنسور الکتروشیمیایی برای شناسایی حساس و انتخاب­پذیر نیتریک اکسید (NO) بر مبنای نانوکامپوزیت گرافن اکسید کاهش یافته الکتروشیمیایی (ErGO)- پلی آنیلین (PANI)- نانوذرات طلا (AuNPs) ساخته شد. این نانوکامپوزیت از طریق الکترودیپوزیشن گام به گام روی یک الکترود طلا (Au) پوشش داده شد تا الکترود AuNPs-PANI-ErGO/Au تشکیل شود. نانوکامپوزیت AuNPs-PANI-rGO به وسیله تکنیک­های UV-vis و FESEM تعیین مشخصه شد. رفتار الکتروشیمیایی الکترود اصلاح شده به وسیله ولتامتری چرخه­ای (CV) و کرونوآمپرومتری (CA) ارزیابی شد. نمودارهای ولتاگرام الکترودهای AuNPs-PANI-ErGO/Au, PANI-ErGO/Au and ErGO/Au نشان داد که هدایت AuNPs-PANI-ErGO/Au از دو مورد دیگر بیشتر بود. از نفیون برای بهبود انتخاب­پذیری الکترود اصلاح شده استفاده شد. الکترود Nafion/AuNPs-PANI-ErGO/Au در مورد اکسیداسیون NO رفتار الکتروکاتالیتیکی و الکتروشیمیایی مطلوبی از خود نشان داد. در شرایط بهینه، الکترود ساخته شده حساسیت بالای μA/μM 113/0 در محدوده خطی وسیع μM 86-8/0 با حد تشخیص پایین μM 25/0 را از خود نشان داد. علاوه بر این، این سنسور پایداری، تکرارپذیری و انتخاب­پذیری بالایی داشت که باعث می­شود بتوان از آن در تشخیص سریع و دقیق NO استفاده کرد.


1.      Moncada, S., Palmer, R. and Higgs, E., "Nitric oxide: Physiology, pathophysiology, and pharmacology", Pharmacological Reviews,  Vol. 43, No. 2, (1991), 109-142.

2.      Pashai, E., Darzi, G.N., Jahanshahi, M., Yazdian, F. and Rahimnejad, M., "An electrochemical nitric oxide biosensor based on immobilized cytochrome c on a chitosan-gold nanocomposite modified gold electrode", International Journal of Biological Macromolecules,  Vol. 108, (2018), 250-258.

3.      Pluth, M.D., Tomat, E. and Lippard, S.J., "Biochemistry of mobile zinc and nitric oxide revealed by fluorescent sensors", Annual Review of Biochemistry,  Vol. 80, (2011), 333-355.

4.      Kavya, R., Saluja, R., Singh, S. and Dikshit, M., "Nitric oxide synthase regulation and diversity: Implications in parkinson’s disease", Nitric Oxide,  Vol. 15, No. 4, (2006), 280-294.

5.      Li, C.M., Zang, J., Zhan, D., Chen, W., Sun, C.Q., Teo, A.L., Chua, Y., Lee, V. and Moochhala, S., "Electrochemical detection of nitric oxide on a swcnt/rtil composite gel microelectrode", Electroanalysis,  Vol. 18, No. 7, (2006), 713-718.

6.      Wink, D.A. and Mitchell, J.B., "Chemical biology of nitric oxide: Insights into regulatory, cytotoxic, and cytoprotective mechanisms of nitric oxide", Free Radical Biology and Medicine,  Vol. 25, No. 4, (1998), 434-456.

7.      Taha, Z.H., "Nitric oxide measurements in biological samples", Talanta,  Vol. 61, No. 1, (2003), 3-10.

8.      Shibuki, K., "An electrochemical microprobe for detecting nitric oxide release in brain tissue", Neuroscience Research,  Vol. 9, No. 1, (1990), 69-76.

9.      Haruyama, T., Shiino, S., Yanagida, Y., Kobatake, E. and Aizawa, M., "Two types of electrochemical nitric oxide (no) sensing systems with heat-denatured cyt c and radical scavenger ptio", Biosensors and Bioelectronics,  Vol. 13, No. 7, (1998), 763-769.

10.    Fan, C., Chen, X., Li, G., Zhu, J., Zhu, D. and Scheer, H., "Direct electrochemical characterization of the interaction between haemoglobin and nitric oxide", Physical Chemistry Chemical Physics,  Vol. 2, No. 19, (2000), 4409-4413.

11.    Brovkovych, V., Stolarczyk, E., Oman, J., Tomboulian, P. and Malinski, T., "Direct electrochemical measurement of nitric oxide in vascular endothelium", Journal of Pharmaceutical and Biomedical Analysis,  Vol. 19, No. 1, (1999), 135-143.

12.    Wen, W., Chen, W., Ren, Q.-Q., Hu, X.-Y., Xiong, H.-Y., Zhang, X.-H., Wang, S.-F. and Zhao, Y.-D., "A highly sensitive nitric oxide biosensor based on hemoglobin–chitosan/graphene–hexadecyltrimethylammonium bromide nanomatrix", Sensors and Actuators B: Chemical,  Vol. 166, (2012), 444-450.

13.    Chen, D., Feng, H. and Li, J., "Graphene oxide: Preparation, functionalization, and electrochemical applications", Chemical Reviews,  Vol. 112, No. 11, (2012), 6027-6053.

14.    Muthoosamy, K., G Bai, R. and Manickam, S., "Graphene and graphene oxide as a docking station for modern drug delivery system", Current Drug Delivery,  Vol. 11, No. 6, (2014), 701-718.

15.    Bai, R.G., Muthoosamy, K., Zhou, M., Ashokkumar, M., Huang, N.M. and Manickam, S., "Sonochemical and sustainable synthesis of graphene-gold (g-au) nanocomposites for enzymeless and selective electrochemical detection of nitric oxide", Biosensors and Bioelectronics,  Vol. 87, (2017), 622-629.

16.    Ting, S.L., Guo, C.X., Leong, K.C., Kim, D.-H., Li, C.M. and Chen, P., "Gold nanoparticles decorated reduced graphene oxide for detecting the presence and cellular release of nitric oxide", Electrochimica Acta,  Vol. 111, (2013), 441-446.

17.    Casero, E., Alonso, C., Vazquez, L., Petit‐Domínguez, M., Parra‐Alfambra, A., De La Fuente, M., Merino, P., Álvarez‐García, S., De Andrés, A. and Pariente, F., "Comparative response of biosensing platforms based on synthesized graphene oxide and electrochemically reduced graphene", Electroanalysis,  Vol. 25, No. 1, (2013), 154-165.

18.    Lian, W., Liu, S., Yu, J., Li, J., Cui, M., Xu, W. and Huang, J., "Electrochemical sensor using neomycin-imprinted film as recognition element based on chitosan-silver nanoparticles/graphene-multiwalled carbon nanotubes composites modified electrode", Biosensors and Bioelectronics,  Vol. 44, (2013), 70-76.

19.    Wang, R., Yan, K., Wang, F. and Zhang, J., "A highly sensitive photoelectrochemical sensor for 4-aminophenol based on cds-graphene nanocomposites and molecularly imprinted polypyrrole", Electrochimica Acta,  Vol. 121, (2014), 102-108.

20.    Tan, X., Hu, Q., Wu, J., Li, X., Li, P., Yu, H., Li, X. and Lei, F., "Electrochemical sensor based on molecularly imprinted polymer reduced graphene oxide and gold nanoparticles modified electrode for detection of carbofuran", Sensors and Actuators B: Chemical,  Vol. 220, (2015), 216-221.

21.    Schoch, K., Byers, W. and Buckley, L., "Deposition and characterization of conducting polymer thin films on insulating substrates", Synthetic Metals,  Vol. 72, No. 1, (1995), 13-23.

22.    Lin, Y. and Cui, X., "Electrosynthesis, characterization, and application of novel hybrid materials based on carbon nanotube–polyaniline–nickel hexacyanoferrate nanocomposites", Journal of Materials Chemistry,  Vol. 16, No. 6, (2006), 585-592.

23.    Batra, B., Lata, S., Rani, S. and Pundir, C., "Fabrication of a cytochrome c biosensor based on cytochrome oxidase/nio-nps/cmwcnt/pani modified au electrode", Journal of Biomedical Nanotechnology,  Vol. 9, No. 3, (2013), 409-416.

24.    Sha, R., Komori, K. and Badhulika, S., "Graphene–polyaniline composite based ultra-sensitive electrochemical sensor for non-enzymatic detection of urea", Electrochimica Acta,  Vol. 233, (2017), 44-51.

25.    Lata, S., Batra, B., Karwasra, N. and Pundir, C.S., "An amperometric H2O2 biosensor based on cytochrome c immobilized onto nickel oxide nanoparticles/carboxylated multiwalled carbon nanotubes/polyaniline modified gold electrode", Process Biochemistry,  Vol. 47, No. 6, (2012), 992-998.

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