IJE TRANSACTIONS A: Basics Vol. 31, No. 4 (April 2018) 516-523    Article in Press

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A. Farahi, G. D. Najafpour and A. Ghoreyshi
( Received: December 05, 2017 – Accepted in Revised Form: January 04, 2018 )

Abstract    Broomcorn seed (Sorghum vulgare) was used as raw material for bioethanol production. Optimum conditions were obtained from response surface method. Broomcorn seed flour (45 g/l) was treated by alkaline treatment and dual enzymatic hydrolysis (0.7 g/l of α- amylase and 0.42 g/l of amyloglucosidase). The hydrolyzed total sugar of 25.5 g/L was used in conventional bioethanol production (8.1 g/l) using Saccharomyces cerevisiae. Enhanced bioethanol production was performed in membrane bioreactor (MBR) in integrated batch fermentation and membrane pervaporation process. Application of commercial polydimethylsiloxane/polyethyleneterephthalate/polyimide (PDMS/PET/PI) membrane in MBR resulted in ethanol concentration of 10.15 g/l in broth and 70.2 g/l in cold trap of MBR. Cell concentration in broth was increased from 7.2 in conventional fermentation to 9.05 g/l in MBR. In addition, ethanol production in MBR using fabricated membrane having ethanol separation factor of 8.7; ethanol concentration in broth and cold trap were 11.1 and 88.5 g/ l, respectively. Also the cell concentration of 10.2 g/l was obtained in MBR with fabricated membrane. In MBR, surface modified multi wall carbon nano tube (MWCNT) coated on membrane having ethanol separation factor of 10.2, resulted ethanol concentration of 11.9 and 110 g/l in broth and cold trap, respectively. Finally, for MBR using modified membrane the cell concentration of 11.01 g/l was obtained. Based on a comparison study, maximum ethanol separation and yield were obtained for modified membrane having MWCNT and the surface was modified by corona treatment.


Keywords    Bioethanol; Multi Walled Carbon Nano Tube; Polydimethylsiloxane; Polyethersulfune; Composite Membrane; Broomcorn Seed


چکیده    دانه­های جارو (سورگوم جارویی) به عنوان ماده اولیه در تولید بیواتانول استفاده شده است. در شرایط بهینه به دست آمده با استفاده از تکنیک پاسخ سطحی، مقدار g/l45 از دانه­های جاروی آرد شده مورد پیش تیمار قلیایی قرار گرفت و سپس با آنزیم­های آلفاآمیلاز با غلظت g/l 7/0 و آمیلوگلوکوزیداز با غلظت g/l 42/0 هیدرولیز آنزیمی گردید. قند ساده حاصل که دارای غلظت g/l 5/25 بود، در روش سنتی با مخمر Saccharomyces cerevisiae تخمیر شد و g/l 1/8 اتانول به دست آمد. دربیوراکتورغشایی و با استفاده از ترکیب عملیات تخمیر و تکنولوژی غشایی تولید اتانول افزایش یافت. با استفاده از غشای تجاری پلی دی متیل سیلوکسان/ پلی اتیلن ترفتالات/ پلی ایمید (PDMS/PET/PI)، اتانول با غلظت g/l 15/10 در بیوراکتور وg/l 2/70 در کلدترپ بیوراکتورغشایی تولید شد. مقدار رشد سلولی از از g/l 2/7 در تولید سنتی به g/l 05/9 رسید. با استفاده از غشای ساخته شده با فاکتور جداسازی 7/8 مقدار اتانول تولیدی در بیوراکتور غشایی به g/l 1/11 و در کلدترپ بیوراکتورغشایی به g/l 5/88 رسید. مقدار رشد سلولی در این حالت برابر g/l 2/10 بوده است. همچنین با استفاده از غشای پلی دی متیل سیلوکسان ساخته شده و اصلاح سطح شده و پوشیده شده با نانولوله­های کربنی چنددیواره با فاکتور جداسازی 2/10، به تولید اتانولی معادل g/l 9/11 و g/l 110 در بیوراکتور و کلدترپ بیوراکتورغشایی دست یافته شد. رشد سلولی در این حالت g/l 01/11 بود. بنابراین بیشترین اتانول تولید شده و جداسازی شده با استفاده از بیوراکتور غشایی و استفاده از غشای اصلاح سطح شده به وسیله کرونا و پوشیده شده با نانولوله­های کربنی چنددیواره می­باشد.


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