IJE TRANSACTIONS B: Applications Vol. 32, No. 2 (February 2019) 184-192    Article in Press

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F. Ahmadi, A. A. Zinatizadeh, A. Asadi and H. Younesi
( Received: August 16, 2018 – Accepted in Revised Form: January 03, 2019 )

Abstract    In this study, the potential of four different culture selection methods under short-term enrichment time (STE) to accumulate PHA-producing bacteria in mixed activated sludge was compared and the most efficient culture selection method was introduced. This means, PHA-producing microbial community was firstly enriched in a sequencing batch bioreactor (SBR) with four different selection methods including an anaerobic-aerobic process (SBR1), a fully aerobic batch process (SBR2), an uncoupled carbon and nitrogen feeding regime (SBR3) and aerobic/anoxic process (SBR4). In the next step, cellular PHA content was maximized in a fed-batch accumulator. From the obtained results, PHA could be accumulated up to 13.2, 10.8, 22.36, and 6 % (mg-PHA/mg-TSS) in SBR1, SBR2, SBR3, and SBR4, respectively. Uncoupled carbon and nitrogen feeding regime (SBR3) showed the best PHA accumulating ability when acetate was used as feed. Also, the SBR3 was fed by soft-drink industrial wastewater to evaluate the capability of the selected strategy for treating real wastewater, which 13.75% of mg-PHA/mg-TSS was achieved.


Keywords    Polyhydroxyalkanoate, Short-term enrichment time (STE), Acetate, Soft drink industrial wastewater



در این پژوهش، پتانسیل چهار روش مختلف در مدت زمان غنی‌سازی کوتاه جهت انتخاب میکروارگانیسم‌های تولیدکننده بیوپلاستیک زیستی (PHA) در جمعیت میکروبی مقایسه شد و روش با کارآیی مناسب معرفی شد. در این میان جامعه میکروبی تولیدکننده PHA ابتدا در یک بیوراکتور دسته‌ای متوالی (SBR) با چهار روش مختلف گزینش شامل: یک فرآیند هوازی بی‌هوازی (SBR1)، یک فرآیند دسته‌ای کاملاً هوازی (SBR2)، یک کربن نیتروژن رژیم تغذیه (SBR3) و فرآیند هوازي/آنوکسی (SBR4) انتخاب شد. در مرحله بعد، محتوای سلولی PHA در یک راکتور تجمع PHA، بیشینه شد. از نتایج به دست آمده، مقدار PHA انباشته شده در SBR1، SBR2، SBR3 و SBR4به ترتیب 2/13، 8/10، 36/22 و 6% (mg-PHA / mg-TSS) می‌باشد. رژیم تغذیه کربن و نیتروژن مجزا (SBR3) بهترین تجمع PHA را (زمانی که استات به عنوان خوراک استفاده شد) نشان داد. همچنين SBR3 با استفاده از فاضلاب صنعتی کارخانه نوشابه‌سازی تغذيه شد تا توانايي استراتژي انتخاب‌شده براي تصفيه فاضلاب واقعي ارزیابی شود كه 75/13% mg-PHA / mg-TSS به دست آمد.


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