IJE TRANSACTIONS B: Applications Vol. 32, No. 2 (February 2019) 184-192    Article in Press

PDF URL: http://www.ije.ir/Vol32/No2/B/1-3000.pdf  
downloaded Downloaded: 197   viewed Viewed: 330

F. Ahmadi, A. A. Zinatizadeh, A. Asadi and H. Younesi
( Received: August 16, 2018 – Accepted in Revised Form: January 03, 2019 )

Abstract    In this study, the potential of four different culture selection methods under short-term enrichment time (STE) to accumulate PHA-producing bacteria in mixed activated sludge was compared and the most efficient culture selection method was introduced. This means, PHA-producing microbial community was firstly enriched in a sequencing batch bioreactor (SBR) with four different selection methods including an anaerobic-aerobic process (SBR1), a fully aerobic batch process (SBR2), an uncoupled carbon and nitrogen feeding regime (SBR3) and aerobic/anoxic process (SBR4). In the next step, cellular PHA content was maximized in a fed-batch accumulator. From the obtained results, PHA could be accumulated up to 13.2, 10.8, 22.36, and 6 % (mg-PHA/mg-TSS) in SBR1, SBR2, SBR3, and SBR4, respectively. Uncoupled carbon and nitrogen feeding regime (SBR3) showed the best PHA accumulating ability when acetate was used as feed. Also, the SBR3 was fed by soft-drink industrial wastewater to evaluate the capability of the selected strategy for treating real wastewater, which 13.75% of mg-PHA/mg-TSS was achieved.


Keywords    Polyhydroxyalkanoate, Short-term enrichment time (STE), Acetate, Soft drink industrial wastewater



در این پژوهش، پتانسیل چهار روش مختلف در مدت زمان غنی‌سازی کوتاه جهت انتخاب میکروارگانیسم‌های تولیدکننده بیوپلاستیک زیستی (PHA) در جمعیت میکروبی مقایسه شد و روش با کارآیی مناسب معرفی شد. در این میان جامعه میکروبی تولیدکننده PHA ابتدا در یک بیوراکتور دسته‌ای متوالی (SBR) با چهار روش مختلف گزینش شامل: یک فرآیند هوازی بی‌هوازی (SBR1)، یک فرآیند دسته‌ای کاملاً هوازی (SBR2)، یک کربن نیتروژن رژیم تغذیه (SBR3) و فرآیند هوازي/آنوکسی (SBR4) انتخاب شد. در مرحله بعد، محتوای سلولی PHA در یک راکتور تجمع PHA، بیشینه شد. از نتایج به دست آمده، مقدار PHA انباشته شده در SBR1، SBR2، SBR3 و SBR4به ترتیب 2/13، 8/10، 36/22 و 6% (mg-PHA / mg-TSS) می‌باشد. رژیم تغذیه کربن و نیتروژن مجزا (SBR3) بهترین تجمع PHA را (زمانی که استات به عنوان خوراک استفاده شد) نشان داد. همچنين SBR3 با استفاده از فاضلاب صنعتی کارخانه نوشابه‌سازی تغذيه شد تا توانايي استراتژي انتخاب‌شده براي تصفيه فاضلاب واقعي ارزیابی شود كه 75/13% mg-PHA / mg-TSS به دست آمد.


1. Morgan-Sagastume, F., Hjort, M., Cirne, D., Gérardin, F., Lacroix, S., Gaval, G., Karabegovic, L., Alexandersson, T., Johansson, P., and Karlsson, A., “Integrated production of polyhydroxyalkanoates (PHAs) with municipal wastewater and sludge treatment at pilot scale”, Bioresource Technology,  Vol. 181, (2015), 78–89.
2. Mumtaz, T., Yahaya, N.A., Abd-Aziz, S., Yee, P.L., Shirai, Y., and Hassan, M.A., “Turning waste to wealth-biodegradable plastics polyhydroxyalkanoates from palm oil mill effluent – a Malaysian perspective”, Journal of Cleaner Production,  Vol. 18, No. 14, (2010), 1393–1402.
3. Majone, M., Dircks, K., and Beim, J.J., “Aerobic storage under dynamic conditions in activated sludge processes. The state of the art”, Water Science and Technology,  Vol. 39, No. 1, (1999), 61–73.
4. Rhu, D.H., Lee, W.H., Kim, J.Y., and Choi, E., “Polyhydroxyalkanoate (PHA) production from waste”, Water Science and Technology,  Vol. 48, No. 8, (2003), 221–228.
5. Dionisi, D., Carucci, G., Papini, M.P., Riccardi, C., Majone, M., and Carrasco, F., “Olive oil mill effluents as a feedstock for production of biodegradable polymers”, Water Research,  Vol. 39, No. 10, (2005), 2076–2084.
6. Bengtsson, S., Werker, A., Christensson, M., and Welander, T., “Production of polyhydroxyalkanoates by activated sludge treating a paper mill wastewater”, Bioresource Technology,  Vol. 99, No. 3, (2008), 509–516.
7. Dionisi, D., Beccari, M., Di Gregorio, S., Majone, M., Papini, M.P., and Vallini, G., “Storage of biodegradable polymers by an enriched microbial community in a sequencing batch reactor operated at high organic load rate”, Journal of Chemical Technology & Biotechnology,  Vol. 80, No. 11, (2005), 1306–1318.
8. Takabatake, H., Satoh, H., Mino, T., and Matsuo, T., “Recovery of biodegradable plastics from activated sludge process”, Water Science and Technology,  Vol. 42, No. 3–4, (2000), 351–356.
9. Liu, C., Liu, D., Qi, Y., Zhang, Y., Liu, X., and Zhao, M., “The effect of anaerobic–aerobic and feast–famine cultivation pattern on bacterial diversity during poly-β-hydroxybutyrate production from domestic sewage sludge”, Environmental Science and Pollution Research,  Vol. 23, No. 13, (2016), 12966–12975.
10. Satoh, H., Iwamoto, Y., Mino, T., and Matsuo, T., “Activated sludge as a possible source of biodegradable plastic”, Water Science and Technology,  Vol. 38, No. 2, (1998), 103–109.
11. Mino, T., van Loosdrecht, M.C.M., and Heijnen, J.J., “Microbiology and biochemistry of the enhanced biological phosphate removal process”, Water Research,  Vol. 32, No. 11, (1998), 3193–3207.
12. Chua, A.S., Takabatake, H., Satoh, H., and Mino, T., “Production of polyhydroxyalkanoates (PHA) by activated sludge treating municipal wastewater: effect of pH, sludge retention time (SRT), and acetate concentration in influent”, Water Research,  Vol. 37, No. 15, (2003), 3602–3611.
13. Salehizadeh, H., and Van Loosdrecht, M.C.M., “Production of polyhydroxyalkanoates by mixed culture: recent trends and biotechnological importance”, Biotechnology Advances,  Vol. 22, No. 3, (2004), 261–279.
14. Beccari, M., Majone, M., Massanisso, P., and Ramadori, R., “A bulking sludge with high storage response selected under intermittent feeding”, Water Research,  Vol. 32, No. 11, (1998), 3403–3413.
15. Dionisi, D., Majone, M., Tandoi, V., and Beccari, M., “Sequencing Batch Reactor:  Influence of Periodic Operation on Performance of Activated Sludges in Biological Wastewater Treatment”, Industrial & Engineering Chemistry Research,  Vol. 40, No. 23, (2001), 5110–5119.
16. Kumar, M.S., Mudliar, S., Reddy, K., and Chakrabarti, T, “Production of biodegradable plastics from activated sludge generated from a food processing industrial wastewater treatment plant”, Bioresource Technology,  Vol. 95, No. 3, (2004), 327–330.
17. Johnson, K., Jiang, Y., Kleerebezem, R., Muyzer, G., and van Loosdrecht, M.C.M., “Enrichment of a Mixed Bacterial Culture with a High Polyhydroxyalkanoate Storage Capacity”, Biomacromolecules,  Vol. 10, No. 4, (2009), 670–676.
18. Johnson, K., Kleerebezem, R., and van Loosdrecht, M.C.M., “Influence of the C/N ratio on the performance of polyhydroxybutyrate (PHB) producing sequencing batch reactors at short SRTs”, Water Research,  Vol. 44, No. 7, (2010), 2141–2152.
19. Johnson, K., Kleerebezem, R., and van Loosdrecht, M.C.M., “Influence of ammonium on the accumulation of polyhydroxybutyrate (PHB) in aerobic open mixed cultures”, Journal of Biotechnology,  Vol. 147, No. 2, (2010), 73–79.
20. Khumwanich, P., and Napathorn, S., “Polyhydroxyalkanoate production with a feast/famine feeding regime using sludge from wastewater treatment plants of the food and beverage industry”, Journal of Biobased Materials and Bioenergy ,  Vol. 8, No. 6, (2014), 641–647.
21. Oliveira, C.S.S., Silva, C.E., Carvalho, G., and Reis, M.A., “Strategies for efficiently selecting PHA producing mixed microbial cultures using complex feedstocks: Feast and famine regime and uncoupled carbon and nitrogen availabilities”, New Biotechnology,  Vol. 37, (2017), 69–79.
22. APHA, Standard methods for the examination of water and wastewater, American Public Health Association, and American Water Works Association, (2005).
23. Braunegg, G., Sonnleitner, B., and Lafferty, R.M., “A rapid gas chromatographic method for the determination of poly-?-hydroxybutyric acid in microbial biomass”, European Journal of Applied Microbiology and Biotechnology,  Vol. 6, No. 1, (1978), 29–37.
24. Wen, Q., Chen, Z., Tian, T., and Chen, W., “Effects of phosphorus and nitrogen limitation on PHA production in activated sludge”, Journal of Environmental Sciences,  Vol. 22, No. 10, (2010), 1602–1607.
25. Kasemsap, C., and Wantawin, C., “Batch production of polyhydroxyalkanoate by low-polyphosphate-content activated sludge at varying pH”, Bioresource Technology,  Vol. 98, No. 5, (2007), 1020–1027.
26. Bengtsson, S., “The utilization of glycogen accumulating organisms for mixed culture production of polyhydroxyalkanoates”, Biotechnology and Bioengineering,  Vol. 104, No. 4, (2009), 698–708.
27. Dionisi, D., Majone, M., Papa, V., and Beccari, M., “Biodegradable polymers from organic acids by using activated sludge enriched by aerobic periodic feeding”, Biotechnology and Bioengineering,  Vol. 85, No. 6, (2004), 569–579.
28. Md Din, M.F., Ujang, Z., van Loosdrecht, M.C.M., Ahmad, A., and Sairan, M.F., “Optimization of nitrogen and phosphorus limitation for better biodegradable plastic production and organic removal using single fed-batch mixed cultures and renewable resources”, Water Science and Technology,  Vol. 53, No. 6, (2006), 15–20.
29. Huang, L., Chen, Z., Wen, Q., and Lee, D.-J., “Enhanced polyhydroxyalkanoate production by mixed microbial culture with extended cultivation strategy”, Bioresource Technology,  Vol. 241, (2017), 802–811.
30. Campanari, S., Augelletti, F., Rossetti, S., Sciubba, F., Villano, M., and Majone, M., “Enhancing a multi-stage process for olive oil mill wastewater valorization towards polyhydroxyalkanoates and biogas production”, Chemical Engineering Journal,  Vol. 317, (2017), 280–289.
31. Ahmadi, F., Zinatizadeh, A.A., and Asadi, A., “PHA production from wastewater by mixed microbial culture under short-term microbial enrichment”, Journal of Applied Research in Water and Wastewater,  Vol. 5, No. 1, (2018), 389–391.
32. Guo, Z., Chen, Z., Wen, Q., Huang, L., Bakke, R., and Du, M., “Strategy to reduce the acclimation period for enrichment of PHA accumulating cultures”, Desalination and Water Treatment,  Vol. 57, No. 60, (2016), 29286–29294.

Download PDF 

International Journal of Engineering
E-mail: office@ije.ir
Web Site: http://www.ije.ir