IJE TRANSACTIONS A: Basics Vol. 32, No. 1 (January 2019) 1-8    Article in Press

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N. Jalilian, G. D. Najafpour and M. Khajouei
( Received: November 18, 2018 – Accepted: January 03, 2019 )

Abstract    Microalgae Chlorella Vulgaris is enriched in vitamin B12 and cobalt ion which is positioned in the center of this vitamin molecule. This study aimed to investigate how different concentrations of cobalt chloride salt affected the vitamin B12 production by utilizing gas, to assess C. vulgaris biomass.Therefore, 0.5, 1.5, 2 (BBM medium) and 2.5 μM cobalt chloride salt was added to C. vulgaris culture medium. Under four cobalt chloride salt treatments, the best growth rate was obtained at the 2 μM of cobalt chloride salt (0.186 + 0.07 g /L.d). gas was supplied by 5% gas cylinder and fermented milk as a novel biological gas generator (10% ). Using fermented milk is a practical approach for elimination of waste gas emission and converting into biomass. The results revealed that, in the presence of 5% gas, C. vulgaris vitamin B12 content at 2 and 2.5 μM cobalt chloride, were 166.23 ± 1.78 and 173.32 ± 4.23 μg /100 g of dry biomass, respectively. However, under controlled condition (ambient air and 2 μM cobalt chloride) vitamin B12 content was 154.9 ± 1.14 μg / 100 g of dry biomass.


Keywords    Chlorella Vulgaris; Vitamin B12; Fermented milk; Biomass


چکیده    will do it at publication stage

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