IJE TRANSACTIONS A: Basics Vol. 32, No. 1 (January 2019) 1-9    Article in Press

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N. Jalilian, G. D. Najafpour and M. Khajouei
( Received: November 18, 2018 – Accepted in Revised Form: January 03, 2019 )

Abstract    Microalgae Chlorella Vulgaris is enriched in vitamin B12 and cobalt ion which is positioned in the center of the vitamin molecule. This study aimed to investigate how different concentrations of cobalt chloride salt affected the vitamin B12 production by utilizing CO2 gas, to assess C. vulgaris biomass. Therefore, Bold’s basal medium used as the medium and 0.5, 1.5, 2 and 2.5 μM cobalt chloride salt was added to C. vulgaris culture. Under four cobalt chloride salt treatments, the best growth rate was obtained at the 2 μM of cobalt chloride salt (0.186 + 0.07 g /L.d). CO2 gas was supplied by 5% CO2 gas cylinder and fermented milk as a novel biological CO2 gas generator (CO2,10%). Use of fermented milk is a practical approach for elimination of waste gas emission and converting CO2 into biomass. The results revealed that, in the presence of 5% CO2 gas, C. vulgaris vitamin B12 content at 2 and 2.5 μM cobalt chloride, were 166.23 ± 1.78 and 173.32 ± 4.23 μg /100 g of dry biomass (7 and 12% higher than control), respectively. However, under controlled condition (ambient air and 2 μM cobalt chloride) vitamin B12 content was 154.9 ± 1.14 μg / 100 g of dry biomass.


Keywords    Chlorella Vulgaris, Vitamin B12, Fermented Milk, Biomass



ریزجلبک کلرلا ولگاریس غنی از ویتامین B12 می¬باشد و یون کبالت در مرکز این ویتامین قرار دارد. این مطالعه با هدف بررسی تاثیرغلظت¬های مختلف نمک کلرید¬کبالت بر تولید ویتامین B12 و تولید زیست¬توده کلرلا ولگاریس در حضور گاز CO2 انجام پذیرفت. بنابراین محیط-کشت BBM جهت کشت این ریزجلبک به کار برده شد و غلظت¬های 5/0، 5/1، 2 و 5/2 میکرومولار از نمک کبالت¬کلرید به محیط کشت اضافه شد. تحت تیمار چهار نمک کلرید¬کبالت، بهترین میزان رشد در 2 میکرومولار نمک کبالت-کلرید حاصل شد (186/0+0.07 گرم بر لیتر در روز). در این پژوهش گاز CO2 توسط سیلند گاز CO2 با غلظت 5% و شیر تخمیر شده به عنوان یک مولد جدید گاز CO2 (CO2 ، 10٪) تامین گشت. استفاده از شیر تخمیر شده یک رویکرد عملی برای از بین بردن انتشار گازهای گلخانه ای و تبدیل گاز CO2 به زیست توده است. نتایج نشان داد، ویتامین B12 تولید شده در حضور گاز CO2 با غلظت 5% و غلظت-های 2 و 5/2 میکرومولار نمک کبالت کلرید به ترتیب78/1± 23/166 و 23.4 ± 32/173 میکروگرم بر 100 گرم زیست¬توده خشک(به ترتیب 7 و 12% بیشتر از شرایط کنترل) می-باشند. این در حالیست که در شرایط کنترل ( هوا و 2 میکرومولار کبالت¬کلرید) 14/1 ± 9/154 میکروگرم B12 به ازای 100 گرم زیست¬توده خشک تولید گشت.


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