IJE TRANSACTIONS B: Applications - Special Issue - Sustainable Technologies for Water and Environment; Guest Editor Prof. Dr. Ahmad Fauzi Ismail, UTM, Malaysia
Vol. 31, No. 5 (May 2018) 884-892    Article in Press

downloaded Downloaded: 0   viewed Viewed: 11

( Received: December 13, 2017 – Accepted: April 18, 2018 )

Abstract    Renewable fuels have received substantial attention as an alternative for fossil fuels due to environmental concern, energy security and diversity. Biofuel is known to be a cleaner and sustainable fuel compared to fossil fuels making it more attractive to be used as transportation fuel. Isobutanol, a four carbon alcohol has several characteristics such as can be blended with gasoline without engine modification, high energy density, low vapour pressure and insoluble in water. The production of isobutanol by Saccharomyces cerevisiae is reported only in small quantity. In order to enhance the production of isobutanol, experiments on effects of medium were conducted in this study. Seven critical nutrients affecting isobutanol production were screened using fractional factorial design. The nutrients involved were glucose, (NH4)2SO4, yeast extract, peptone, KH2PO4, MgSO4.7H2O and FeSO4.7H2O. A total of 70 experiments including six replication of centre points were conducted. The screening gave three significant mediums including glucose, peptone and yeast extract. The significant factors were further optimized using central composite design (CCD) and response surface methodology (RSM) with the range of 80-140 g/L, 4-8 g/L and 4-8 g/L, respectively. From the experimental results, optimum isobutanol concentration of 172 mg/L was obtained at the optimum condition of glucose (140 g/L), peptone (8 g/L) and yeast extract (8 g/L) which is 87% higher compared to unoptimized medium compositions.


Keywords    Biofuel, Isobutanol, Optimization, RSM, Saccharomyces cerevisia


References    [1] Sanchez-Ramirez E., Quiroz-Ramirez J.J., Segovia-Hernandez J.G., Hernandez S. and Bonilla-Petriciolet A., “Process alternatives for biobutanol purification: design and optimization”, Industrial and Engineering Chemistry Research, Vol. 54, (2015), 351-358.  [2] Kumar M. and Gayen K., “Development in biobutanol production: new insights”, Applied Energy, Vol. 88, (2011), 1999-2012. [3] Hazrat M.A., Rasul M.G. and Khan M.M.K., “Biofuel: An Australian perspective in abating the fossil fuel vulnerability”, Procedia Engineering, Vol. 105, (2015), 628-637.  [4] Gonela V. and Zhang J., “Design of the optimal industrial symbiosis system to improve bioethanol production”, Journal of Cleaner Production, Vol. 64, (2014), 513-534. [5] Dahman Y. “Sustainable biobutanol and working towards the green gasoline of the future”, Fermentation Technology, Vol. 1, No. 3, ,( 2012), DOI: 10.4172/2167-7972.1000e111. [6] Milne, N. Wahl, S.A. van Maris, A.J.A. Pronk, J.T. and Daran, J.M., “Excessive by-product formation: A key contributor to low isobutanol yields of engineered Saccharomyces cerevisiae strains”, Metabolic Engineering Communications, Vol. 3, (2016), 39-51. [7] Lee, W.-H. Seo, S.-O. Bae, Y.-H. Nan, H. Jin, Y.-S. and Seo, J.-H., “Isobutanol produxtion in engineered Saccharomyces cerevisiae by overexpression of 2-ketoisovalerate decarboxylase and valine biosynthetic enzymes”, Bioprocess and Biosystem Engineering, (2012.). [8] Ghiaci, P., Norbeck, J. and Larsson, C., “Physiological adaptations Saccharomyces cerevisiae evolved for improved butanol tolerance”, Biotechnology Biofuels, Vol. 6, (2013), 101. [9] Jin C., Yao M., Liu H., Lee C. F. and Ji J., “Progress in the production and application of n- butanol as a biofuel”, Renewable and Sustainable Energy Reviews, Vol. 15, (2011.), 4080- 4106. [10] Brat, D. Weber, C. Lorenzen, W. Bode, H.B. and Boles, E., ”Cytosolic re-localization and optimization of valine synthesis and catabolism enables increased isobutanol production with the yeast Saccharomyces cerevisiae”, Biotechnology Biofuels, Vol. 5, (2012), 65. [11] Kondo, T. Tezuka, H. Ishii, J. Matsuda, F. Ogino, C. and Kondo, A., “Genetic engineering to enhance the ehrlich pathway and alter carbon flux for increased isobutanol production from glucose by Saccharomyces cerevisiae”,  Journal of Biotechnology, Vol.159, (2012), 32-37. [12] Hahn- Hagerdal, B., Karhumaa, K., Larsson, C. U., Gorwa- Grauslund, M., Gorgens, J. and van Zyl, W. H., “Role of cultivation media in the development of yeast strains for large scale industrial scale”, Microbial Cell Factories Vol. 4, (2005), 31. [13] Huang, W. C. and Tang, I. C., “Bacterial and yeast cultures-process characteristics, products and applications. In Yang, S.-T. (Ed.) Bioprocessing for value-added products from renewable resources”,Oxford, UK: Elsevier, (2007). [14] Bharati, T., Kulkarni, J.H., Krishnaraj, P.U. and Alagawadi A.R., “Effect of different carbon sources on the biomass of Metarhizium anisopliae (Ma2)*”, Karnataka Journal of Agricultural Science, Vol. 20, No. 2, (2007), 310-311 [15] Li, Z., Wang, D. and Shi, Y.-C., “Effects of nitrogen source on ethanol production in very high gravity fermentation of corn starch”, Journal of theTaiwan Institute of Chemical Engineers, Vol. 70, (2017), 229-235. [16] Zheng, J., Tashiro, Y., Zhao, T., Wang, Q., Sakai, K.and Sonomoto, K., “Enhancement of acetone-butanol-ethanol fermentation from eucalyptus hydrolysate with optimized nutrient supplementation through statistical experimental designs”, Renewable Energy, Vol. 113, (2017), 580-586. [17] Ranjan, A., Mayank, R. and Moholkar, V. S.,” Process optimization for butanol production from developed rice sraw hydrolysate using Clostridium acetobutylicum MTCC 481 strain”, Biomass Conversion and Biorefinery, Vol. 3, (2013), 143-155. [18] Bezerra, M. A., Santelli, R. E., Oliveira, E.P., Villar, L. S. and Escaleira, L.A., “Response surface methodology (RSM) as a tool for optimization in analytical chemistry”, Talanta, Vol. 76, (2008), 956-977. [19] Wang, Y. and Blaschek, H. P., “Optimization of butanol production from tropical maize stalk juice by fermentation with Clostridium beijerinckii NCIMB 8052”, Bioresource Technology, Vol. 102, (2011), 9985-9990.  [20] Moon, C., Lee, C. H., Sang, B.-I. and Um, Y., “Optimisation of medium compositions favoring butanol and 1, 3-propanediol production from glycerol by Clostridium pasteurianum”, Bioresource Technology, Vol. 102, (2011), 10561-10568. [21] da Porto, C., Voinovich, D., Decorti, D. and Natolino, A., “Response surface optimization of hemp seed (cannabis sativa l.) oil yield and oxidation stability by supercritical carbon dioxide extraction”, Journal of Supercritical Fluids, Vol. 68, (2012), 45-51. [22] Stanbury, P. F. and Whitaker, A., “The Development of inocula for industrial fermentations. In Hall, S. J. (Ed.) Principles of Fermentation Technology”, Oxford: Pergamon, (1984). [23] Kampen, W. H., “Nutritional requirements in fermentation process. In: Fermentation and biochemical engineering handbook”, Edited by Vogel, H. C. and Todaro, C. M. 3rd edition. Elsevier Inc, (2014). [24] Kim, J.-H., Roy, A., Jouandot, D. and Cho, K. H., “The glucose signaling network in yeast”, Biochimica et Biophysica Acta, Vol. 1830, (2013), 5204-5210. [25] Carlson, M. “Glucose repression in yeast”, Current Opinion in Microbiology, Vol. 2, (1999), 202-252. [26] Roy, A., Kim, Y.-B., Cho, K. H. and Kim, J.-H., “Glucose starvation-induced turnover of the yeast glucose transporter Hxt1”,  Biochimica et Biophysica Acta, Vol. 1840, (2014), 2878-2885. [27] Al-Shorgani, N. K., Hamid, A. A., Yusoff, W. M. W. and Kalil, M. S., “Pre-optimization of medium for biobutanol production by a new isolate of solvent-producing Clostridium”, BioResources, Vol. 8, No. 1, (2013), 1420-1430. [28] Singh, K. G., Lapsiya, K. L., Gophane, R. R. and Ranade, D. P., “Optimization for butanol production using Plackett-Burman Design coupled with Central Composite Design by Clostridium beijerinckii strain CHTa isolated from distillery waste manure”,J. Biochem. Tech., Vol. 7, No. 1, (2016), 1063-1068. [29] Acourene, S. and Ammouche, A., “Optimization of ethanol, citric acid, and α-amylase production from date wastes by strains of Saccharomyces cerevisiae, Aspergillus niger and Candida guilliermondii”, Journal of Industrial Microbiology and Biotechnology, Vol. 39, (2012), 759-766. [30] Deesuth, O., Laopaiboon, P., Jaisil, P. and Laopaiboon, L., “Optimization of nitrogen and metal ions supplementation for very high gravity bioethanol fermentation from sweet sorghum juice using an orthogonal array design”, Energies, Vol. 5, (2012), 3178-3197.   [31] Mechmech, F., Marinova, M., Chadjaa, H., Rahni, M., Akacha, N., B. and Gargouri, M., “Alfalfa juice as a nitrogen source or supplement for acetone-butanol-ethanol production by Clostridium acetobutylicum”, Industrial Crops and Products., Vol. 78, (2015), 73-81. [32] Wechgama, K., Laopaiboon, L. and Laopaiboon, P., “Enhancement of batch butanol production from sugarcane molasses using nitrogen supplementation integrated with gas stripping for product recovery”,. Industrial Crops and Products., Vol. 95, (2017), 216-226

International Journal of Engineering
E-mail: office@ije.ir
Web Site: http://www.ije.ir