Abstract




 
   

IJE TRANSACTIONS B: Applications - Special Issue - Sustainable Technologies for Water and Environment; Guest Editor Prof. Dr. Ahmad Fauzi Ismail, UTM, Malaysia
Vol. 31, No. 5 (May 2018) 884-892    Article in Press

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  OPTIMIZATION OF MEDIUM COMPOSITIONS FOR ISOBUTANOL PRODUCTION BY SACCHAROMYCES CEREVISIAE USING RESPONSE SURFACE METHODOLOGY
 
N. A. RAMLI and R. A. RAHMAN
 
( Received: December 13, 2017 – Accepted: April 18, 2018 )
 
 

Abstract    Renewable fuels have received substantial attention as an alternative for fossil fuels due to environmental concern, energy security and diversity. Biofuel is known to be a cleaner and sustainable fuel compared to fossil fuels making it more attractive to be used as transportation fuel. Isobutanol, a four carbon alcohol has several characteristics such as can be blended with gasoline without engine modification, high energy density, low vapour pressure and insoluble in water. The production of isobutanol by Saccharomyces cerevisiae is reported only in small quantity. In order to enhance the production of isobutanol, experiments on effects of medium were conducted in this study. Seven critical nutrients affecting isobutanol production were screened using fractional factorial design. The nutrients involved were glucose, (NH4)2SO4, yeast extract, peptone, KH2PO4, MgSO4.7H2O and FeSO4.7H2O. A total of 70 experiments including six replication of centre points were conducted. The screening gave three significant mediums including glucose, peptone and yeast extract. The significant factors were further optimized using central composite design (CCD) and response surface methodology (RSM) with the range of 80-140 g/L, 4-8 g/L and 4-8 g/L, respectively. From the experimental results, optimum isobutanol concentration of 172 mg/L was obtained at the optimum condition of glucose (140 g/L), peptone (8 g/L) and yeast extract (8 g/L) which is 87% higher compared to unoptimized medium compositions.

 

Keywords    Biofuel, Isobutanol, Optimization, RSM, Saccharomyces cerevisia

 

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