IJE TRANSACTIONS B: Applications Vol. 31, No. 11 (November 2018) 1803-1809    Article in Press

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S. Rezaei, M. Mohammadi, G. D. Najafpour, A. Moghadamnia, S. Kazemi and M. Nikzad
( Received: June 09, 2018 – Accepted in Revised Form: August 17, 2018 )

Abstract    Curcumin is the natural bio-active component of turmeric (Curcuma longa L.) with known therapeutic properties; nevertheless, its biological applications are limited due to its poor bioavailability. To overcome this limitation, curcumin was conjugated with silica nanoparticles. Curcumin was separated from turmeric by microwave-assisted extraction and silica nanoparticles were developed from rice husk. Conjugation of curcumin with silica nanoparticles was performed through ultrasound-assisted wet impregnation. XRD, FTIR and UV-visible analyses confirmed the successful synthesis of the conjugate; the drug loading in the nanoparticle was 39% as determined by HPLC analysis. TEM and AFM analyses indicated the spherical morphology of the conjugate with average particle size of 85.9 nm. The cell killing activity of the conjugate was tested against HeLa, MCF-7 and Saos-2 cancer cell lines and normal fibroblast cell line using MTT assay. The silica:curcumin conjugate was effective for destruction of cancerous cells, especially HeLa cells, with minimum side effects on healthy fibroblasts.


Keywords    Curcumin; Silica Nanoparticles; Bioavailabilit; Anti-cancer Activity


چکیده    کورکومین ترکیب زیست‌فعال طبیعی موجود در زردچوبه (کورکوما لونگا) با اثرات درمانی شناخته شده است؛ با این حال، کاربرد بیولوژیکی آن با توجه به زیست‌فراهمی پائینی که دارد محدود است. به منظور غلبه بر این محدودیت، کورکومین با نانوذرات سیلیکا ترکیب شد. کورکومین با استخراج به کمک مایکروویو از زردچوبه جداسازی شد و نانوذرات سیلیکا از شلتوک برنج تولید شدند. ترکیب کورکومین با نانوذرات سیلیکا با روش خیساندن مرطوب با کمک امواج فراصوت انجام شد. آنالیزهای XRD، FTIR و امواج مرئی-فرابنفش سنتز موفق این ترکیب را تایید کردند؛ بارگذاری دارو روی نانوذره 39% بود که با آنالیز HPLC تعیین گردید. آنالیزهای TEM و AFM نشان دادند که مورفولوژی نانوذرات کروی بوده و متوسط اندازه ذرات 9/85 نانومتر بود. خاصیت ضدسرطانی ترکیب سنتز شده روی سلول‌های HeLa، MCF-7 و Saos-2 و همچنین سلولهای فیبروبلاست نرمال با استفاده از تست MTT مورد بررسی قرار گرفت. نتایج نشان داد که ترکیب کورکومین:سیلیکا روی سلول‌های سرطانی، به خصوص سلول‌هایHeLa ، اثر مخرب داشت درحالی که کمترین اثر جانبی را روی سلول‌های فیبروبلاست سالم نشان داد.


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